Today done my second RNA Isolation
But seem like no a really good isolation
A lot of DNA Contamination appear
Read back the troubleshooting article
I think my problem was on the homogenization step
Maybe does not mix well after adding Trizol
Forget to incubate for 5 min at room temperature after homogenization?
Or insufficient volume of Trizol
Something wrong with my technique when pipetted out the Trizol?
Tomorrow will proceed to quantification step
Hope that there will be some sample fulfill the standard of RNA quantification (concentration and purity)
Realize that it is actually not much time for me to waste anymore in this step
This should be a simple step
Have to get the starting sample (RNA Stock) done quickly and proceed to subsequent step
Wish me Good Luck !
Really Cannot just simply do a thing without have basic knowledge on it
If u know the principle behind the technique
Is possible for u to troubleshoot the difficulties
5 comments:
It's alrite. Practice makes perfect. Better to be slow but steady than to rush and worry.
Miss Ma..thanks for ur encouragement ~
yaya...really practice makes perfect..at the beginning i even not sure how to use pipette..evything hav to learn, practice..go through d try n error process..learn from mistakes..is a learning process..will be alrite at the end
there's a book in CAIS, 'the research student's guide to success' u might wanna give it a read. since u r just starting research, quite interesting and maybe useful.
All right~
I will try to get it and read through~
thanks~ ^-^
Unfortunately, the book u mention above was hold by somebody.. I think maybe is lecturer.. because due date is till 06-05-2011...
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